Functional genomics resources for the North Atlantic copepod, Calanus finmarchicus: EST database and physiological microarray.
Animals, Atlantic Ocean, Copepoda, Copepoda: genetics, Copepoda: metabolism, Copepoda: physiology, databases, Expressed Sequence Tags, Female, Gene Expression Profiling, Gene Library, Genes, Genes: genetics, genetic, Genomics, Genomics: methods, Metabolic Networks and Pathways, Oligonucleotide Array Sequence Analysis, Transcriptome
The copepod, Calanus finmarchicus is a keystone species for the North Atlantic. Because of recent changes in the geographic distribution of this species, there are questions as to how this organism responds physiologically to environmental cues. Molecular techniques allow for examination and new understanding of these physiological changes. Here, we describe the development of a microarray for high-throughput studies of the physiological ecology of C. finmarchicus. An EST database was generated for this species using a normalized cDNA library derived from adult and sub-adult individuals. Sequence data were clustered into contigs and annotated using Blastx. Target transcripts were selected, and unique, 50 base-pair, oligomer probes were generated for 995 genes. Blast2GO processing provided detailed information on gene function. The selected targets included broad representation of biological processes, cellular components, and molecular functions. The microarray was tested in two sets of comparisons: adult females maintained at different food concentrations and field-caught sub-adults showing differences in lipid storage. Up-regulated and down-regulated transcripts were identified for both comparisons. Only a small subset of the genes up-regulated in low food individuals were also up-regulated in lipid-poor animals; no overlap was seen between the genes down-regulated in the two comparisons.